cell nuclear antigen (Proteintech)
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Proteintech
cell nuclear antigen
Cell Nuclear Antigen, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+nuclear+antigen+pcna/PCNA+Antibody/pmc12830253-61-13-17
Average 96 stars, based on 1337 article reviews
Cell Nuclear Antigen, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+nuclear+antigen+pcna/PCNA+Antibody/pmc12830253-61-13-17
Average 96 stars, based on 1337 article reviews
cell nuclear antigen - by Bioz Stars,
2026-09
96/100 stars
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Binding Assay:Article Title: Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression. Article Snippet: .. Non-specific binding was blocked with 1% skim milk/PBS for 15 min. Primary antibodies for cytokeratin 19 (CK19) (Proteintech Group, Wuhan, China, 1:100), cytokeratin 7 (CK7) (Proteintech Group, Wuhan, China, 1:500), or proliferating Article Title: The USP8/CEP55/CHMP6 Axis Orchestrates Triple-Negative Breast Cancer Progression by Regulating Ferroptosis and Macrophage M2 Polarization. Article Snippet: Our work establishes the USP8/CEP55/CHMP6 signaling cascade as a critical driver of TNBC malignancy, highlighting its potential as a therapeutic target.. Future studies should aim to develop novel inhibitors targeting this axis to sensitize TNBC cells to ferroptosis and reverse immunosuppression, thereby improving treatment outcomes.. Moreover, investigating the interplay between this pathway and other oncogenic signaling networks may uncover additional opportunities for combinatorial therapies in TNBC. Article Title: Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression Article Snippet: .. Nonspecific binding was blocked with 1% skim milk/PBS for 15 min. Primary antibodies for cytokeratin 19 (CK19) (Proteintech Group, Wuhan, China, 1:100), cytokeratin 7 (CK7) (Proteintech Group, Wuhan, China, 1:500), or proliferating Incubation:Article Title: Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression. Article Snippet: .. Non-specific binding was blocked with 1% skim milk/PBS for 15 min. Primary antibodies for cytokeratin 19 (CK19) (Proteintech Group, Wuhan, China, 1:100), cytokeratin 7 (CK7) (Proteintech Group, Wuhan, China, 1:500), or proliferating Article Title: The USP8/CEP55/CHMP6 Axis Orchestrates Triple-Negative Breast Cancer Progression by Regulating Ferroptosis and Macrophage M2 Polarization. Article Snippet: Our work establishes the USP8/CEP55/CHMP6 signaling cascade as a critical driver of TNBC malignancy, highlighting its potential as a therapeutic target.. Future studies should aim to develop novel inhibitors targeting this axis to sensitize TNBC cells to ferroptosis and reverse immunosuppression, thereby improving treatment outcomes.. Moreover, investigating the interplay between this pathway and other oncogenic signaling networks may uncover additional opportunities for combinatorial therapies in TNBC. Article Title: Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression Article Snippet: .. Nonspecific binding was blocked with 1% skim milk/PBS for 15 min. Primary antibodies for cytokeratin 19 (CK19) (Proteintech Group, Wuhan, China, 1:100), cytokeratin 7 (CK7) (Proteintech Group, Wuhan, China, 1:500), or proliferating Article Title: Unveiling the role of Jagged2 in hypoxic pulmonary arterial hypertension: A NOX2‐mediated pathway Article Snippet: .. The membrane was blocked with 5% nonfat dry milk at room temperature for 1 h. The PVDF membrane was incubated overnight at 4°C with primary antibodies diluted in tris‐buffered saline with tween 20 (TBST): Jag2 (bs‐4244R, Bioss), NOX2 (19013‐1‐AP, Proteintech), nuclear factor erythroid 2‐related factor 2 (Nrf2) (16396‐1‐AP, Proteintech), proliferating Article Title: L-tetrahydropalmatine attenuates methamphetamine-induced pulmonary vascular remodeling via CaSR-BMP2/mTOR signaling. Article Snippet: The World Symposium on Pulmonary Hypertension (WSPH) raised a new classification, “Drugand toxininduced pulmonary arterial hypertension (PAH)”, in which methamphetamine (MA) is recognized as an important contributor.. L-tetrahydropalmatine (L-THP), an alkaloid with analgesic and sedative properties, has demonstrated vascular protective effects, but its mechanism against MA-induced pulmonary injury remains unclear.. The calcium-sensing receptor (CaSR) regulates multiple cellular processes, including proliferation, differentiation, migration, adhesion, and apoptosis, and may participate in MA-related pulmonary vascular remodeling. Gentle:Article Title: Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression. Article Snippet: .. Non-specific binding was blocked with 1% skim milk/PBS for 15 min. Primary antibodies for cytokeratin 19 (CK19) (Proteintech Group, Wuhan, China, 1:100), cytokeratin 7 (CK7) (Proteintech Group, Wuhan, China, 1:500), or proliferating Article Title: Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression Article Snippet: .. Nonspecific binding was blocked with 1% skim milk/PBS for 15 min. Primary antibodies for cytokeratin 19 (CK19) (Proteintech Group, Wuhan, China, 1:100), cytokeratin 7 (CK7) (Proteintech Group, Wuhan, China, 1:500), or proliferating Multiple Displacement Amplification:Article Title: FGF4 alleviates the lung cell damage caused by high glucose via AMPK-PGC-1 signaling axis in vitro and in vivo Article Snippet: Cell culture plates, FBS, BSA and TBST buffer were from Beyotime Institute of Biotechnology. .. CyclinD1, proliferating Membrane:Article Title: Unveiling the role of Jagged2 in hypoxic pulmonary arterial hypertension: A NOX2‐mediated pathway Article Snippet: .. The membrane was blocked with 5% nonfat dry milk at room temperature for 1 h. The PVDF membrane was incubated overnight at 4°C with primary antibodies diluted in tris‐buffered saline with tween 20 (TBST): Jag2 (bs‐4244R, Bioss), NOX2 (19013‐1‐AP, Proteintech), nuclear factor erythroid 2‐related factor 2 (Nrf2) (16396‐1‐AP, Proteintech), proliferating Saline:Article Title: Unveiling the role of Jagged2 in hypoxic pulmonary arterial hypertension: A NOX2‐mediated pathway Article Snippet: .. The membrane was blocked with 5% nonfat dry milk at room temperature for 1 h. The PVDF membrane was incubated overnight at 4°C with primary antibodies diluted in tris‐buffered saline with tween 20 (TBST): Jag2 (bs‐4244R, Bioss), NOX2 (19013‐1‐AP, Proteintech), nuclear factor erythroid 2‐related factor 2 (Nrf2) (16396‐1‐AP, Proteintech), proliferating |
![hfNCSC-sEVs are taken up by PCs in vitro and enhance their proliferation and migration. (A) Primary cultures of hfNCSCs were established from male Sprague–Dawley rats. (B) Immunofluorescence staining of the neural crest cell marker p75 (red) and the stem cell marker nestin (green) in hfNCSCs, with 4′,6-diamidino-2-phenylindole (DAPI) staining indicating the nuclei. (C) Western blot analysis demonstrated the presence of surface markers (cluster of differentiation [CD]9, CD81, and tumor susceptibility gene 101 protein [TSG101]) and the absence of an endoplasmic reticulum marker (calnexin) in hfNCSC-sEVs. (D) Nanoparticle tracking analysis was used to quantify the concentration and size distribution of hfNCSC-sEVs. (E) Transmission electron microscopy was used to visualize the characteristic morphology of hfNCSC-sEVs. (F) Immunofluorescence staining indicated that the third-generation PCs cultured in vitro were positive for claudin-1, zonula occludens 1 (ZO1), and glucose transporter 1 (GLUT1) but negative for S100, with DAPI staining marking the nuclei. (G) The internalization of PKH26-labeled hfNCSC-sEVs (red) by ZO1-positive PCs (green) was visualized using immunofluorescence staining, with DAPI staining to mark the nuclei. (H) The Cell Counting Kit-8 assay was used to evaluate the cell viability of PCs across concentrations of 0, 2 × 10 8 , 5 × 10 8 , and 10 × 10 8 particles/mL hfNCSC-sEVs at 3, 5, and 7 days of in vitro culture ( n = 5 per group). (I) The Transwell assay was used to quantify the number of migrating PCs at 6, 12, and 18 hours post-treatment with the aforementioned concentrations of hfNCSC-sEVs, in in vitro culture ( n = 6 per group). (J) Western blot and (K) statistical analyses revealed the relative protein expression levels of proliferating cell nuclear antigen <t>(PCNA)</t> and vimentin in PCs from the phosphate-buffered saline (PBS) and hfNCSC-sEVs groups on day 5 of in vitro culture (normalized to β-actin, n = 3 per group). Data are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance and Tukey’s multiple comparison test for H and I; Student’s t -test for K). The data were from at least three separate and independent studies. CCK-8: Cell counting kit-8; GLUT1: glucose transporter 1; hfNCSCs: hair follicle neural crest stem cells; ns: not significant; PCNA: proliferating cell nuclear antigen; PCs: perineurial cells; sEVs: small extracellular vesicles; ZO1: zonula occludens 1.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4726/pmc12694726/pmc12694726__NRR-21-2060-g002.jpg)
